The following protocols are for performing colorimetric-based nutrient analyses on a microplate reader. Protocols developed and aggregated for the MacDougall, McCann, and Rooney labs at UofG.

Nutrient Protocols

Water samples

Ammonium - aqueous

Nitrate - aqueous

Orthophosphate - aqueous

Total phosphorus - aqueous

Dissolved organic carbon - aqueous

Soil samples

Nitrate + Ammonium - soil

Inorganic phosphorus - soil

Total phosphorus - soil

General protocols or scripts

Acid bath

Computer, Epoch

Script for analysis in R

Nutrient name ID template

Nutrient plate layout template

UofG restricted forms

Please email me if you need access to the forms! The link will only work if you have been granted access, thanks!

Lab Chemical Inventory

Cost-sharing and usage tracking

Useful links

Soil Protocols --> Field and Laboratory Procedures Used in a Soil Chronosequence Study, USGS, edited by Michael J. Singer, Peter Janitzky

Plant Tissue %P