The following protocols are for performing colorimetric-based nutrient analyses on a microplate reader. Protocols developed and aggregated for the MacDougall, McCann, and Rooney labs at UofG.

Nutrient Protocols


Dissolved organic carbon



Total phosphorus

General protocols or scripts

Acid bath

Computer, Epoch

Script for analysis in R

Nutrient name ID template

Nutrient plate layout template

Useful links

Soil Protocols --> Field and Laboratory Procedures Used in a Soil Chronosequence Study, USGS, edited by Michael J. Singer, Peter Janitzky

Plant Tissue %P